DNA Technology

 

How to make a "prokaryotic protein factory"

            Background:

                        Viruses can infect living cells

                        Bacteria produce restriction enzymes to cut up viral DNA

                        Bacteria take up plasmids

            Technique:

                        Cut out source DNA with restriction enzyme

            Open up plasmid with same restriction enzyme

                        Mix "prepared" DNA and plasmids

                        Mix plasmids with plasmid-less bacteria

                        Kill off other bacteria

                        Culture remaining bacteria